本文筛选了日本沼虾细胞培养的最适培养基：以M199（Gibco）为基础培养基,添加20％胎牛血清（FBS）,青霉素100IU/mL,链霉素100μg/mL,1g/L葡萄糖,5.2g/LNaCl,1.43g/LCaCl_2,0.05g/LMgCl_2,0.2g/LNaHCO_3.以此培养基对日本沼虾肌肉和肝胰腺进行了原代细胞培养,井将肌肉细胞传代3次. 实验中发现,幼虾比成虾的组织更易培养成活.另外细胞培养与季节和蜕皮周期有关,春季虾优于夏秋季虾；蜕皮后12小时内的沼虾比蜕皮间期沼虾的组织更易培养成活.

    本实验以春季第一批日本沼虾幼虾为实验材料,在沼虾蜕皮后12内进行接种,取得了较好的培养效果.肝胰腺细胞存活8-15天,肌肉细胞被传代3次,存活54天. 本文以RNA/DNA、总磷和无机磷含量为生理和生化指标,分别测定了几种不同渗透压（480、570、650mmol/kg）和pH值（6.8、7.2、7.6、8.0、8.4、8.8）条件下体外培养肌肉细胞的生长状况.指出570mmol/kg为培养细胞的最适渗透压,在此渗透压条件下.培养细胞的RNA/DNA最大,而总磷含量最小（P＜0.05）.培养细胞的适宜pH范围是7.2-8.0,其最适pH为7.6,在此pH条件下,传代细胞的RNA/DNA和总磷含量均最大（P＜0.05）. 

    在此基础上,本文研究了微量元素锌对体外培养肌肉细胞的影响,结果表明0-60μg/L Zn~(2+)能促进传代肌肉细胞的生长,Zn~(2+)60μg/L组的细胞生长最快,RNA/DNA值和总磷含量最大（P＜0.05）,这说明培养基中Zn~(2+)浓度在60μg/L为宜.过量的Zn~(2+)则表现出重金属的毒性作用.

    Primary shrimp cell cultures were developed from muscle, hepatopand and hemocytes of Macro brachium nipponense in medium 199 supplemented with 20% fetal bovine serum(FBS), 5.2g/L NaCI, I .43g/LCaCI2, 0.05g/LMgC12, 100IU/mL penicillin, 100 ii g/mL streptomycin. Basal medium supplemented with 1 g/L glucose could enhance growth and cells performance. Muscle cells were passaged up to three times. It was found that better results for cell culture would be obtained more easily with the juvenile shrimp caught in spring than the adults in summer or autumn; and shrimps gotten within 12 hours after ecdysis could grow much better than the intermoult shrimps. The osmolality and pH of the different media were tested. The results showed that the optimum conditions for cell culture in vitro were obtained in medium 199 with an osmolality of approximately 57ommol/kg. The favorable pH range of the muscle cells was pH 7.2-8.0, with an optimum of pH7.6. In another way, basal medium supplemented with Zn260 tt g/L could enhance the growth of the muscle cells, The highest concentrations of total phosphorus and the largest RNA/DNA values were found in the groups of Zn~(2+) 60 ii g/L.