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基于历史实验研究的啮齿类动物的造血干细胞被界定其生物学特性：高表达某些表面抗原（如CD34） ，以及不表达特异性标记（如，DR） 。最近研究结果表明，造血重建可以从骨髓、外周血和脐血细胞分离获得CD34-的干细胞。 CD34-干细胞被认为是静态造血干细胞和间充质干细胞的主要部分。由于新技术， CD34-的干细胞可分化为真正水平上的干细胞，还能分化成特定的组织或器官。这就需要在体外建立原代成纤维细胞样CD34 -干细胞，以及细胞永生化的完善系统。
Isolation of Primary and Immortalized CD34– Hematopoietic and Mesenchymal Stem Cells from Various Sources
Based on historical radiation experiments in rodents, the hematopoietic stem cell was defined by its biological properties and later by the expression of certain surface antigens (e.g., CD34), as well as the absence of lineage-specific markers (e.g., DR). Quite recently it was shown that hematopoietic reconstitution can also be achieved by CD34– stem cells, which can be isolated from the bone marrow, peripheral blood and cord blood cells. CD34– stem cells are considered to be predominately part of the quiescent stem cell pool of hematopoietic and mesenchymal stem cells. Due to novel techniques, CD34– stem cells can be expanded on the level of a true stem cell but also directed towards their differentiation into specified tissues or organ systems. This requires the establishment of primary fibroblast-like CD34– stem cells in vitro and their possible reversible and transient immortalization with optimized vector systems.
