TOE1作为Egr1的靶位点而被发现.他们一起参与通过上调P21表达调节的细胞增殖和G2/M时期的细胞周期延迟.我们发现TOE1能够结合P53抑癌蛋白,特别是通过P53 c 端相互作用形成四聚体结构.通过光干扰测定法测定结合动力学进一步鉴定了他们的相互作用,同时发现这个相互作用能够增强基于P53靶基因的转录活性.这些结果表明TOE1是p53的共调节子.

TOE1 interacts with p53 to modulate its transactivation potential.
    Sperandio S, Tardito S, Surzycki A, Latterich M, de Belle I.
    Centre de Recherche du CHUL, Department of Molecular Endocrinology and Oncology, Laval University, Quebec, QC Canada.
    The TOE1 gene was discovered as a target of the Egr1 transcription factor that participates in cell growth regulation through the upregulation of p21 and a cell cycle delay at the G2/M phase. We report here that TOE1 is able to bind to the p53 tumor suppressor protein, specifically interacting with the C terminal tetramerization domain of p53. We have further characterized this interaction through determination of binding kinetics using nanoporous optical interferometry and demonstrated that this interaction is capable of enhancing the transcriptional activity of p53-dependent gene targets. These results suggest a mechanistic role for TOE1 as a co-regulator of p53.
PMID: 19508870 [PubMed - in process]

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