Ly-6蛋白超家族是一类含有LU结构域的蛋白,LU结构域大约有80个氨基酸,该结构的特征为具有一个保守的10个半胱氨酸残基序列.在本研究中我们从人睾丸cDNA文库中分离并鉴定了一个新的含有LU结构域的基因LYPD6,通过UCSC基因组数据库搜索将该基因定位于2q23.1-23.2.LYPD6 cDNA长3501bp,编码171个氨基酸.在COS-7细胞中过表达LYPD6,亚细胞定位显示它在细胞质表达.RT-PCR分析发现LYPD6在人类组织中广泛表达,同时在心脏和脑中表达相对较高.另外,HEK 293T细胞中过表达LYPD6,基于报道基因分析表明它能够抑制AP1的转录活性.

  1: Mol Biol Rep. 2009 Aug 4. [Epub ahead of print]
  Identification and characterization of human LYPD6, a new member of the Ly-6 superfamily.
  Zhang Y, Lang Q, Li J, Xie F, Wan B, Yu L.
  State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Handan Road 220, 200433, Shanghai, People\'s Republic of China.
  The Ly-6 protein superfamily is usually identified as a group of proteins with a LU protein domain. LU domain is about 80 amino acids long and characterized by a conserved pattern of 10 cysteine residues. Here we report the cloning and characterization of a novel human LU domain containing gene, LYPD6, isolated from human testis cDNA library, and mapped to 2q23.1-23.2 by searching the UCSC genomic database. The LYPD6 cDNA sequence of 3,501 base pairs contains an open reading frame encoding 171 amino acids. Subcellular localization of LYPD6 demonstrated that the protein was localized in the cytoplasm when overexpressed in COS-7 cells. RT-PCR analysis showed that LYPD6 was widely expressed in human tissues and the expression levels in brain and heart were relatively high. Furthermore, the subsequent analysis based on reporter gene assays suggested that overexpression of LYPD6 in HEK 293T cells was able to suppress the transcriptional activities of AP1.

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